8 Pages. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 00 USD. The planktonic growth inhibition (%) was calculated according to the following equation:Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. – ™FilterMax F5 Multi-Mode Microplate Reader (cat. FilterMax F5 Multi-Mode Microplate Reader. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-The next day, precultures were diluted to an optical density of 0. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. A microplate reader (also called sometimes microtiter plate reader) is a high-sensitivity instrument designed to analyze samples contained in microplate wells. 4. Methods for MycoAlert and MycoAlert PLUS Assays are basically the same, except for the concentration range of assay control used, and are detailed below. Before unpacking and setting up the FilterMaxTM F3 or FilterMaxTM F5 Microplate Reader, prepare a dry, flat work area that has sufficient space for the instrument, host computer,. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumFlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell. The capability of the lixiviated products of the experimental membranes to inhibit the bacterial planktonic growth was estimated from the turbidity of the inoculated culture media at OD λ = 595 nm (filter max F5 multi-mode microplate reader). 8 Pages. BMG LABTECH. Synergy HTX laboratory equipment pdf manual download. The amount of colorimetric product was continuously monitored for 120 min. 3. Click Run. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. Absorbance Detection Comparison Table. 5. MS-3 Laser Bar Code Reader. Activation of TLR4 and TLR2 receptors. SpectraMax M-series Microplate readers. The experiment was carried out with four biological replicates and two technical replicates. Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader. Serum concentrations of CAF were determined according to manufacturer guidelines in duplicate via enzyme-linked immunosorbent assay (Glory Science Co. 00FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. 3. The FilterMax F3 features absorbance (visible), fluorscence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. SpectraMax Paradigm Multi-Mode Microplate Reader. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. 2. The ELISA test was performed using the supernatants from the morphological test. FlexStation 3 Multi-Mode Microplate Reader. GFP expression differences. com A. 5. The OD 595 of the culture in the plate (light path length: ∼6. SpectraMax Gemini XPS/EM Microplate Readers. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. 00 USD. A blank sample was prepared by mixing. Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat SYRINGE/VALVE CHAMBER FOR RQF INSTRUMENT quench flow analysis KIN-TEK RAPID QUENCH FLOW INSTRUMENT KIN-TEK RQF-3 VALVE/SYRINGE CHAMBER Biorad Laboratory Real Time PCR System& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. In . For 3D cell proliferation assay, (high concentrations) in 3D cellular proliferation assay, 20, 100 or 500 µg/ ml MG were used. 030 fmol Fluorescence Polarization (FP). com 8. SpectraMax MiniMax 300 Imaging Cytometer for the SpectraMax i3x Multi-Mode Microplate Detection Platform KEY FEATURES • ombine cellular imaging C and microplate assays into a single workflow • liminate cell staining for cell E counting and confluency with StainFree Technology • omplete cell viability C and toxicity assays with. Figure 1. SoftMax® Pro Microplate Data Analysis Software provides integrated instrument control and data analysis. The solution was incubated for 15 min and fluorescence measured on a SpectraMax microplate reader (Molecular Devices) with an. Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). In addition, the FilterMax F5 Reader is capable of measuring nucleic acids directly at 260 nm in absorbance detection mode. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Samples were considered positive if the average sample absorbance was greater than twice the mean absorbance of healthy. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. S. SpectraMax Quant dsDNA Assay Kits. Transfluor Technology. Assay buffer and 100% DMSO were used as vehicle controls. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se The method is based on the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA) under conditions of high temperature and acidity that generates a fluorochrome, which is quantified by fluorimetry (F5 Multi-Mode Microplate Reader), with excitation at 515 nm and emission at 553 nm. Multi-Mode microplate readers offer flexibility and ease of use over a broad range of applications. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Yes, the M5 does have a limited slip. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. Mazumder, in Laser Additive Manufacturing, 2017 1. Instrument Configuration: Assay Technology Adapta® & FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell-based application requirements while. The colorimetric signal development was stopped after 10 minutes. 2 Pages. , San Diego, CA, USA). 2. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). For FcγRI (high affinity receptor) binding assays, plates were coated with 1 μg/mL recombinant human soluble. Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. Guiberteau MD, in Essentials of Nuclear Medicine Imaging (Sixth Edition), 2012 Photon Transducers. com Check our website for a current listing SpectraMax i3/i3x Multi-Mode Microplate Reader SpectraMax Paradigm Multi-Mode Microplate Reader MOLECULAR DEVICES The trademarks used herein are the. Match case Limit results 1 per page. #0200-7030) Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. GPCRs. F5 has made an exception to this policy for BIG-IP 13. They provide absorbance, fluorescence, glow luminescence, and other read modes for Affordability and unmatched assay performance in filter-based microplate readers. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. A photomultiplier tube (PMT) converts a light pulse into an electrical signal of measurable magnitude. 4 million by 2027; it is expected to grow at a CAGR of 3. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). Transfluor Technology. The MTT assay was performed with 100 μM vitamin E in the presence or absence of LPS at a concentration of 1 µg/mL for 24 h. The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. Thereafter, the dye liquid was removed, the formazan was solubilized with dimethyl sulfoxide (DMSO) under continuous agitation for 15 min, and the absorbance of each well was read by the means of a microplate reader (FilterMax F5 Multimode Microplate Reader; Molecular Devices) at a wavelength of 570 µm. MetaFluor Software. The fluorescence was determined using a fluorimetric microplate reader (FilterMax F5 Multi-Mode Microplate Reader) at 540 and 595 nm for excitation and emission, respectively. HTRF-compatible SpectraMax Multi-Mode Readers. Figure 2-23: Bottle holder To install the bottle holder: 1. The quantity of the colorimetric product was monitored continuously for 120 min. , Sunnyvale, CA, USA) and normalized to the absorbance in the. Click to Contact Seller. Absorbance was detected from the bottom using the FilterMax F5 multimode microplate reader (Molecular Devices, Sunnyvale, CA) at excitation/emission wavelengths of 485 nm/535 nm. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. Instrumentation and Quality Control. OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader with high sensitivity. 3. Upgradeable modules are also available including western blot, cell imaging, and fast. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. 4. Cells were co-transfected with 2 μg total plasmid DNA (1760 ng empty vector or proviral plasmid DNA. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. MetaXpress. ImageXpress Micro 4 High-Content Imaging System. Please click on the links below to view the Microplate Reader Comparison Tables. 40266 Email: drugdiscoverytech@lifetech. 7°C (gradient heating design). Plate Formats:. 0´ 104 1. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. 8 Pages. 5 mP. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. Fred A. FilterMax™ F3 and FilterMax™ F5 Multi-Mode Microplate Reader User Guide 5008528 A September 2010 DTX Multimode Readers User Guide. Fold changes were calculated as the area under the curve of stimulated HKLs relative to unstimulated HKLs (RPMI without zymosan). All experiments were performed in triplicate. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. MetaMorph NX. MetaMorph NX. The samples were transferred to black 96-well plates and fluorescence was measured at an excitation wavelength of 405 nm and an emission wavelength of 535 nm on the FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices) equipped with the SoftMaxPro Microplate Data Acquisition and Analysis 6. 97059-524EA 84727. Molecular Devices Multi-Mode Microplate Reader FilterMax F5. Luciferase activity was quantified by the Dual-Glo Luciferase Assay System (Promega) as per the manufacturer’s instructions on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices). SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 713579). A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. , Sunnyvale, CA, USA). The hemolysis was calculated with the following formula: Hemolysis (%) = (A sample − A PBS)/(A Triton − A PBS. Absorbance microplate assays For more than 30 years, absorbance-based detection has been the preferred mode for many microplate-based assays, such as ELISA, protein, and nucleic acid quantitation or enzymatic assays. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. FilterMax Multi-Mode Microplate Readers Functionality FilterMax F3 FilterMax F5 Absorbance (ABS ) Visible 4 4 Absorbance (ABS) UV 4 Fluorescence intensity (FI) top read 4 4 Fluorescence intensity (FI) bottom read 4 Luminescence (LUM) glow 4 4 Fluorescence polarization (FP) 4 Time-Resolved fluorescence (TRF) 4 Shaking 4 4 Temperature. #ID5-STD), equipped with the following: Enhanced TRF Module (Molecular Devices cat. The drawing shows a standard microplate of 15 mm height covered with a lid. time plots at 30, 60, and 90 min using MATLAB® R2017b (9. 3. A) CHO colonies, serum-free suspension-adapted, in CloneMedia-CHO. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. BIO TEK Synergy? 2 Multi-Detection Microplate Reader with Luminescence and Dual Reagent Dispenser SPECTRA MAX 190 MICROPLATE READER (90V TO 240V) WITH CURRENT BMG FLUOSTAR OPTIMA PLATE READER Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat FilterMax F5 Multi-Mode Microplate Reader. Synergy™ H1 Hybrid Multi-Mode Microplate Reader, Wavelength Range: 200 nm to 999 nm, BioTek Instruments (CAT#:. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The measured temperature can be used to provide feedback. Topic. Select the protocol “Concentration_pico_green_v3. Absorbance values were normalized on the control at 24 h and analyzed as being proportional to the number of viable cells. MetaMorph NX. Dave Zeckhausen. SpectraMax Glo Steady-Luc Reporter Assay Kit. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. The 50% inhibitory concentration (IC 50) values were determined using the nonlinear regression curve fit analysis of Prism 9. Each kit was used on the same day that its reagents were reconstituted. Page: 1/4 NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. FilterMax F3 & F5 Microplate Readers. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. Technical specifications Compatible with these Molecular Devices systems Contact Us Phone: +1-800-635-5577 Email: [email protected]. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of. Depending on the model, Cytation 5 detection modes include fluorescence intensity (FI), fluorescence polarization (FP), time-resolved fluorescence (TRF), luminescence, UV-visible absorbance, imaging, and alpha. FilterMax F3 and F5 Multi-Mode Microplate Readers; Absorbance Readers. 3 Pages. The optical performance is comparable to a top-of-the-line dedicated Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. The experiment was carried out with four biological replicates and two technical replicates. The OD 600 was plotted against time using Graphpad Prism 8. c. 3 Pages. #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). 3 Pages. 3 Pages. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. Page 4 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 5. Multi-Mode Microplate Reader. Add to cart. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Load prepared PicoGreen plate (step C. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. ”SpectraMax® Paradigm® Multi-Mode Detection Platform. S. Supplier: Molecular Devices. These assays span over a wide wavelength range, generally from 220-900 nm in different detection modes (typically absorbance, fluorescence and luminescence). The standard curve presented a linear phase from 1. BioStack Neo is a dedicated stacker offering super-FilterMax™ F3 and FilterMax™ F5 Multi-Mode Microplate Reader User Guide 5008528 A September 2010 DTX Multimode Readers User Guide. Plate Formats:. 425 or. ** Some compatible filter sets are available for purchase from Life. Vascular. 00. F3/F5 Multi-Mode Microplate ReadersThe plate was incubated for 1 hr at room temperature with shaking. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 00The microplate reader market was valued at US$ 534. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. Drug repurposing has been an interesting and cost-effective approach, especially for neglected diseases, such as Chagas disease. Besides the number of substrates used by microbiota,. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 6 reviews. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). Learn More. com B. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). FilterMax F5 Multi-Mode Microplate Reader. Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). The Molecular Devices FilterMax F5 Multi-Mode Plate Reader was tested for compatibility with Life Technologies’ Z'-LYTE® assays using the Z'-LYTE® Tyr6 kit (PV4122) against. 00 USD. A 1:1 binding ratio Hill model was used to fit the binding interaction data. Open SoftMax® Pro software. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 030 fmol Fluorescence Polarization (FP) (Fluorescein 1 nM, SD) 96-well (200 µL) 0. Synergy HTX and Synergy LX. Plaque reduction. Cell growth and viability were assessed using the CCK-8 proliferation assay. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. This document is. Other technologies such as imaging. BioStack 4 offers patented plate de-lidding and re-lidding for sensitive cell-based workflows. Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and. ScanLater Western Blot Detection System. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. CloneMatrix. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. 0. Manufacturer: Molecular Devices Model: FilterMax F5 Condition: Used. Description: The FilterMax™ F3 features absorbance (visible),. , call 1-800-635-5577. 6 Pages. SoftMax Pro GxP Compliance Software. F3/F5 Multi-Mode Microplate Readers. All experiments were performed in triplicate. ABS Test results. MetaFluor Software. Buffer without protease was used as a blank for background subtraction. 005 OD. Cell viability was measured in percentages (%) as compared to untreated cells, which were assumed to be 100% viable. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. An anti-human HER2/neu human IgG3/κ (Huang and Morrison, 2006) was used as isotype (negative) control. This can increase throughput and efficiency, thus saving time and space in the lab. 4 Pages. I. , Ltd. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices Supplier: Molecular Devices. Page 38 | Getting Started Overview The Cytation 5 is a hybrid multi-mode microplate reader. By category. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . MICROPLATE READERS Multi-Mode Readers. Other recommended products. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. Chapter 1: Introduction The FilterMax™ F3 Multi-Mode Microplate Reader and the FilterMax™ F5 Multi-Mode Microplate Reader are ideal for a broad range of applications, including drug discovery, genomics, proteomics, and cell-based research. The absorption was measured in a spectrophotometer ultraviolet–visible (UV–Vis) Filter Max F5 multi-mode microplate reader; a wavelength of 595 nm was taken as the standard value for bacteria. The amount of each HTRA1 protein was. Laser-aided direct metal deposition of metals and alloys. Cells were harvested for luciferase assay 48 h after transfection. 9 mM H 2 O 2 for. The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Protein quantification was carried out using the Pierce™ BCA Protein Assay Kit (Thermo Fisher) and a FilterMax F5 Multi-Mode Microplate Reader (San Jose, CA, United SA). After the mixture was cooled, TBARS was measured at 532 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Reader, Molecular Devices). Preconfigured HTRF protocols in SoftMax ® Pro Software 7. Related Products: FilterMax F3 & F5 Multi-Mode Microplate Readers, SpectraMax i3 Multi-Mode Platform Microplate Detection System,. The next day, precultures were diluted to an optical density of 0. Important things to consider when purchasing a multimode microplate reader. The absorbance values of the results were expressed as optical density (OD) as the mean ± standard deviation. Add. Cells were seeded in 24-well plates and cultured. Catalog. Page 1 SpectraMax® M3 SpectraMax® M4 SpectraMax® M5 SpectraMax® M5e Multi-Mode Microplate Readers User Guide 0112-0115 G October 2018. The donor and acceptor can be used to label a variety of biomolecules, for. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. 5 ml 0. Click “Eject Plate Carrier. 3 Pages. 3 Pages. F5 Multi-Mode Microplate Reader. SpectraMax iD5. 8 Pages. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. ** Some compatible filter sets are available for purchase from Life. An array of these tubes is situated behind the sodium iodide crystal and may. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Products : General, FilterMax F5 reader Please click on the links below to view the Microplate Reader Comparison Tables. 7°C and the bottom to 36. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $3,500. When recombinant supernatants were tested for the presence of Il10, only values within the linear range of. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Your applications, your modes, your choice. Supplier: Molecular Devices. Measurements of Caspase-3/7 Activity. , Sunnyvale, CA, USA). Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Citations: Dual monochromators increase performance and assay flexibility. Related Products: FilterMax F3 &. SpectraMax Glo Steady-Luc Reporter Assay Kit. 40266 Email: drugdiscoverytech@lifetech. The user must evaluate this instrument and PC-based software in conjunction with their specific assay(s). FilterMax F5 Multi-mode microplate reader. Many absorbance assays use a chromogenic substrate, which, upon enzymatic conversion to the final product, results in a compound thatThen 200 µl of 10% (v/v) ammonium hydroxide was added to neutralize the acid and absorbance readings were taken at 405 nm with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices. Equal concentrations of protein were separated in Mini-PROTEAN® TGX™ Precast 4–20% Gels (Bio-Rad, Hercules, CA, USA) and transferred to nitrocellulose. BMG Labtech - THERMOstar. Description Z-height focusing is enabled for fast top- and bottom-reads for all read modes. 3 Pages. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 4 (Molecular Devices, Sunnyvale, CA, USA) at room temperature (fluorimeter settings used were as follows: measurement type, kinetic, 60 cycle, 60-s interval; excitation wavelength, 360 nm;. Generally when this occurs, the destination BIG-IP device is unable to execute the tmsh command successfully. Caspase-3 Activity. 4 Pages. ] Example of document to evaluate if the reader . (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Excitation Filter – EXP1 F535/25, F485/20, F485/20, F370/TRF. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 667 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. Protein contamination can be measured at 280 nm. F3 F5 F3-SC F5-SC F3 F5. Microplate readers, multi-mode, FilterMax™ F3 and F5 Supplier: Molecular Devices Description: The FilterMax™ series of multi-mode microplate readers can be operated as standalone instruments or can be integrated seamlessly with laboratory autom. Supernatant (25 μL), or standard, was added and mixed. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. Transfluor Technology. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Z’-LYTE® Compatible Microplate Reader Documentation Version No. shopping cart 0 items $0. FilterMax F5 Multi-mode microplate reader. RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). In addition, the FilterMax F5 Multi-Mode Microplate Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. FilterMax F3 / FilterMax F5: Inquire: SpectraMax iD3 and iD5: SpectraMax M: Price: Description: Unlimited breadth of user-upgradeable application modules expands research capabilities. Select the protocol “Concentration_pico_green_v3. 2.